Expression profiling of bladder cancer by DNA microarrays allows the identification of non-invasive diagnostic markers

Lourdes Mengual, Moisés Burset, Elisabet Ars, Juan José Lozano, Humberto Villavicencio, María José Ribal, Antonio Alcaraz

Department and Laboratory of Urology and CIBEREHD. Hospital Clínic, Institut Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Barcelona, Spain. Laboratory of Molecular Biology and Service of Urology. Fundació Puigvert, Barcelona, Spain.

Journal of Urology (in press)

Supplementary material

Table 1 List of 3126 probe-sets (corresponding to 2408 different genes) with at least two fold differential expression (log2ratio in absolute value >1) between bladder tumors and control urothelium.

Table 2 List of 674 probe-sets (corresponding to 530 different genes) with at least two fold differential expression (log2ratio in absolute value >1) between HG and LG bladder tumors.

Table 3 Lists of probe-sets indicated in Figure 1B and 1C.

Table 4 Primers/probe set, from the internal Applied Biosystems description, used for gene expression measurements in the qRT-PCR experiments.

Table 5 Gene expression quantification by qRT-PCR in BW of the 6 most differently expressed genes in bladder tumors according to microarray analysis.

Table 6 Gene expression quantification by qRT-PCR in BW of 8 of the most differently expressed genes between high and low grade bladder tumors according to microarray analysis.